K Ananda

Bioactive compounds from plant and endophytic fungal association

Lignin degrading enzymes from endophytic fungi

Chemical modification of therapeutic protein for enhancing their half life

  1. Anti-diabetic compounds of endophytic fungi from Medicinal plants used for the treatment of diabetes:Diabetes is one of major prevailing diseases around the world with high risks of growth in India. Preventing diabetes is the best option at present situation. If at all one gets a diabetes then maintaining normal blood sugar level in the blood is a major task throughout the life. There are various types of medications available for controlling diabetes but each one has one or the other side effects. Natural extracts from medicinal plants or endophytic fungi can be an alternative to avoid such side effects. This  research group focusing on isolating anti-diabetic compounds from the endophytic fungi which are living inside the medicinal plants and study their applications in treating diabetes

  2. Effect of radiation on endophytic fungi producing ligninase enzyme and antidiabetic inhibitors:Radiation exposure can cause different effects on organisms in the environment. There are cases where plants yield was increased by exposing them to different types of radiation. Similarly, if we expose endophytic fungi (which are already known for producing industrially useful enzymes and secondary metabolites for pharmaceutical applications) for various types of radiation that might influence in increased production of ligninase or secondary metabolites by the fungi. As the ligninase is known for degradation of phenolic compounds, can be applied in degradation of textile and other industrial wastes. Secondary metabolites may be useful as inhibitors of enzymes involved in diabetes control.

  3. Modification of Insulin molecule to improve its half-life and other therapeutic properties: Controlling sugar in a diabetic patient has become a major challenge due to increased diagnosis of diabetes cases in recent years. Type 2 diabetes is a major type of diabetes and main causes are insulin resistance, reduced secretion and others. The purpose of this research is to modify the insulin using conjugation chemistry using protein or non-protein molecules to increase the half-life as well as its pharmacodynamics properties in-vitro and/or in-vivo models. 

  1. Deboleena Dhara, Dhanya Sunil, Pooja R Kamath, Ananda Kulal, S. Shrilakshmi and S. Balaji (2017). New Oxadiazole Derivatives: Synthesis and Appraisal of Their Potential as Antimicrobial Agents. Letters in Drug Design & Discovery14(999):1-1.
  2. Isloor, Arun M., Raghavendra Hebbar, Kulal Ananda, Hohd Sohaimia Abdullah, and Ahmad Ismail. "Fabrication of novel hollow fiber membrane decorated with functionalized Fe2O3 nanoparticles: Towards sustainable water treatment and biofouling control." New Journal of Chemistry (2017) (Accepted).
  3. Nandam Harikrishna, Arun M. Isloor, K. Ananda, Tanya Parish, Joazaizulfazli Jamalis, Hazem A. Ghabbour and Hoong-Kun Fun (2017). Antitubercular and Antimicrobial Activity of NH4VO3 Promoted 1,4-Dihydropyridine Incorporated 1,3,4-trisubstituted Pyrazole. Letters in Drug Design & Discovery,  14, 000-000
  4. Poojari, J., Sunil, D., Kamath, P.R., Ananda, K., Kirana, M.P. (2016). Antioxidant Properties and Electrochemical Behavior of some Acetyl Salicylic Acid Derivatives. Journal of Applied Pharmaceutical Science Vol, 6(11), 165-171
  5. Shubhalaxmi, Manjunatha BS, Ananda K, Bhat KS. (2016) Synthesis of a series of pyrazoline derivatives carrying tosyl substitution and evaluation of their antimicrobial properties. J App Pharm Sci 6 (11): 214-217.
  6. Shubhalaxmi, B.S., Ananda, K. and Bhat, K.S., (2016). Pyrimidinethione derivatives with tosyl substitution: Synthesis, and antimicrobial property investigation. Journal of Applied Pharmaceutical Science Vol, 6(06), pp.073-078.
  7. Shubhalaxmi, Pathak L, Ananda K, Bhat KS. (2016): Synthesis of focused library of novel aryloxyacids and pyrazoline derivatives: Molecular docking studies and antimicrobial investigation. Cogent Chemistry, (2):1141388.
  8. Hebbar RS, Isloor AM, Ananda K, Ismail AF. Fabrication of polydopamine functionalized halloysite nanotube/polyetherimide membranes for heavy metal removal. Journal of Materials Chemistry A. 2016;4(3):764-74.N Harikrishna, Arun M Isloor, K Ananda, Abdulrahman Obaid, Hoong-Kun Fun (2015). 1, 3, 4-Trisubstituted pyrazole bearing a 4-(chromen-2-one) thiazole: synthesis, characterization and its biological studies. RSC Advances, 5(54), 43648-43659.
  9. Pavithra N., Sathish L., Suneel Kumar A., Venkatarathanamma, V., Pushpalatha H., Bhanuprakash Reddy, G., Ananda K. (2014): In-vitro Studies on α-Amylase, α-Glucosidase and Aldose Reductase Inhibitors found in Endophytic Fungi Isolated from Ocimum sanctum, Current Enzyme Inhibitor 10(2)129-136.
  10. N. Pavithra,   L. Sathish, Nagasai, Babu,  V. Venkatarathanamma,  H. Pushpalatha,  G. Bhanuprakash Reddy,  K. Ananda (2014) Evaluation of α-Amylase, α-Glucosidase and Aldose Reductase inhibitors in ethyl acetate extracts of endophytic fungi isolated from anti-diabetic medicinal plants.Int J Pharm Sci Res. 5 (12) 5334-5341
  11. Garudachari B, Isloor AM, Satyanaraya MN, Ananda K, Fun H-K. (2014) Synthesis, characterization and antimicrobial studies of some new trifluoromethyl quinoline-3-carbohydrazide and 1,3,4-oxadiazoles. RSC Advances. 2014;4(58):30864-30875
  12. Sathish, L., Pavithra, N. and Ananda K. (2014) Evaluation of antimicrobial activity of secondary metabolites and enzyme production from endophytic fungi isolated from Eucalyptus citriodora. Journal of Pharmacy Research 8(3); 269-276.
  13. Garudachari B, Isloor AM, Satyanarayana MN, Fun HK, Pavithra N and  Ananda K. (2013)Design and regioselective synthesis of trifluoromethylquinolone derivatives as potent antimicrobial agents. European Journal of Medicinal Chemistry 68; 422-432.

Completed Projects:

  1. Antimicrobial and anti oxidants of endophytic fungi from Medicinal plants
    Principal  Investigator: Dr.Ananda K. ; Research  Fellow:  Sathish  L
  2. Anti-diabetic compounds of  endophytic fungi from medicinal plants used for the treatment of diabetes.
    Principal  Investigator: Dr.Ananda K. ; Research  Fellow:  Pavithra N
  3. Anticancer metabolites from endophytes of medicinal plants
    Principal Investigator: Dr.  Ananda K

 

Ongoing Projects:

  1. Effect of electron beam radiation on endophytic fungi producing ligninase enzyme
    PI:Dr.Ananda, K. ; Research Scholar: Ms.Kavitha KN
    Endophytic fungi isolated from Simarouba glauca  were tested for the ligninase enzymes production. Endophytic fungi positive for ligninase are exposed to electron beam radiation and subcultured in to a new media. The effect of various doses of electron beam radiation was analysed with respect to the production of ligninase enzymes. The dye degradation rate was also analysed for all the fungal cultures exposed to radiation. The changes in rate of dye degradation found after irradiation observed in some of the cultures. The cultures were grown for several generations to check the consistence in enzyme production. The dose of radiation at which fungi produces highest amount of enzyme will be used for further studies.
  2. Alpha glucosidase enzyme inhibitors from Simarouba glauca and from its endophytic fungi
    PI: Dr.Ananda K, Reseach Scholar: Mr.Kiran M P
    Alpha glucosidase enzymes are involved in degradation of disaccharides into monosaccharides and these mono saccharides are absorbed into the blood stream in the small intestine. The starchy food taken into the body immediately digested and blood glucose level will increase in the body. The enzyme insulin will be secreted into blood stream and all the excess of glucose in the blood will be stored as glycogen under normal condition. In case of diabetic patient the glucose taken into the blood will be stored immediately due to improper activity of insulin or other receptors. Hence, the diabetic patients are not encouraged to eat starchy food. To avoid this elevation in blood glucose level in the blood the present study focused on searching for a natural alpha glucosidase inhibitor.
  3. Site specific modification of insulin for increasing its half-life and pharmacological properties
    PI: Dr.Ananda K. ; Research Scholar : Ms. Shrilakshmi S
    Insulin protein has chain A and Chain B in the active form. The binding affinities and sites of biological degradation of insulin will be analysed by molecular docking studies. The modification of amino acids either by mutating an less important amino acid with the easily modifiable one or modification of a specific amino acid by a polyethylene glycol molecule to reduce rate of insulin degradation. There will be few different types of molecules will be prepared by such modifications and each one will be analysed for their biochemical properties and later tested for in-vitro models.

  

Research Collaboration

Dr.  Arun M Isloor, Associate Professor, NITK, Surathkal collaboration for the Biological activity of synthesized compounds and materials.

Genelon Institute of  Lifesciences, Yelahanka, Bangalore made a MOU for mutual collaborative research and Dr.Ananda K is a member of scientific advisor committee.

Govt.  Sponsored project:

Received research grant for the project titled “Effect of electron beam radiation on endophytic fungi producing ligninase enzyme” for three years from BRNS, DAE, Govt. of India

2011-Present:  Assistant Professor, PPISR, Bangalore, India

2010-2011:  Faculty Fellow, PPISR, Bangalore, India

2004-2010:  Post-Doctoral Fellow, Albert Einstein College of Medicine, NY, USA

2004-2004:  Lecturer, P A College of Engineering, Mangalore, India

2003-2004: Project   Associate,   IWST, Bangalore, India

1999-2003:   Administrative Supervisor, KSRTC, Govt.  of Karnataka, India

1996-2001:  PhD, Mangalore University, Mangalore, India.

1995-1996:  Project Assistant, College of Fisheries, Mangalore, India

1993-1995:  MSc, Biosciences, Mangalore University, Mangalore, India

Research Collaboration

  1. Dr. Arun M Isloor, NITK, Surathkal collaboration for the antimicrobial assay of synthetic compounds

Govt. Sponsored project:

  1. Received research grant for the project titled "Effect of electron beam radiation on endophytic fungi producing ligninase enzyme" for three years from BRNS, DAE, Govt. of India

Training of Students:

  1. PhD Awarded : 02
  2. MSc Project completed : 03
  3. BSc Project completed : 06
  4. BSc Inspire students : 03
  5. PhD students working : 03

Membership of Professional bodies

  1. Life Member of Mycological Society of India
  2. Life member of Association of Microbiologist of India

Journal review

  1. RSC Advances
  2. BMC complementary and alternative Medicine
  3. Journal of Food Science and Technology
  4. Cogent chemistry

Ms. Kavitha K N

PhD Student

 


 Mr. Kiran M P 

PhD Student

 


Ms. Shrilakshmi S 

PhD Student

 


Dr. Pavithra N.  

Alumni

 


Dr.  Sathish  L

Alumni